Hello chimera team, is there any memory-limitation for the split-map tool? When i perform a segmentation of a density-map larger than ~100MB i always get a chimera-error. In order to perform the segmentation, i have to coarse the density-map with a factor of 2, as example in imagic, and then split-map works fine with the new map. The result is of course density maps with a lower resolution. My Linux-PC has 4GB of memory and my first thought was that this should be enough for the segmentation of the density map as example in 10 parts (10x100=1GB). Is there any way to circumvent this problem and perform the segmentation with the original map? Thanks Christos Gatsogiannis Christos Dipl.Biol. Cryo-EM Lab Institut for Zoology Johannes Gutenberg University 55128, Mainz Germany Tel: +49(0)6131-39-23091