To whom this may concern,

I’m an undergraduate student doing some research at KSTATE this summer and I’ve been assigned to work on a project that utilizes Chimera.

My professor is unavailable a lot of the time and I’ve been struggling a bit with a problem.

I’ve been successful at opening up the following PDB files:

1DHK 1DPX 1HYL 2CAU 3BLG 1OA6 1JBC 1OA5

I’m able to open up the .pdb files, proceed to use the commands “del solvent” and “del @ .b” in the command line.

I’m also able to go to ToolsàHigher Order Structure à Crystal Contacts before changing the contact distance from 1.0 to 4.0 and check mark the create copies of contacting molecules.

However when I attempt to do the following actions for the rest of the proteins I run into a variety of issues:

For example 2CAU:

When I attempt to “measure buried area #0 # 2-7,” I get the following response in my reply log:

#0, chain A: JACK BEAN vicilin

6 pairs of NCS asymmetric units of 2CAU contact at distance 1.0 A

Atoms MTRIX SMTRY Unit cell MTRIXref Copies

1 0 4 0 0 0 0 1

1 0 5 0 0 0 0 1

1 0 6 -1 0 -1 0 1

1 0 8 0 0 0 0 1

1 0 9 0 0 -1 0 1

1 0 11 0 0 -1 0 1

C:\Program Files\Chimera 1.6.1\bin\mscalc.exe 1.400000 2.000000 1