Hello, I could be wrong, but I am not sure whether the other program (Relion?) refers to the Gaussian cube format. Rather, I think it is literally just saying that the generated mask is not a cube-shaped grid (X^3) but a non-cube (XxYxZ). I think Relion for example complains about that. One solution could be to resample the newly generated mask onto your initial grid, e.g. 'vop resample #1 onGrid #0', which will place your mask onto the cube-shaped grid of #0. Best, Moritz On 2/22/21, 11:21, "Chimera-users on behalf of Elaine Meng" <chimera-users-bounces@cgl.ucsf.edu on behalf of meng@cgl.ucsf.edu> wrote: External Email - Use Caution Hi Smith, We are not experts in other programs, so I can only guess: maybe the other program does not want MRC format. There is some other map format called "cube" that Chimera can read (Gaussian cube format in this list: <https://secure-web.cisco.com/17Qgqo7DM_hHO1zGFYtl3CJ8P56TeOIxzPvNxSRxXYTiTTSqQAOlbP7lFUc7NJDjjYsX5ui0bIVFjn5IST0rCUw5-80tFI39LokTva_Zl7iSBrZ-G28aiKIWo-_-6lSbQqMzoZ_-GRh7TA9a6eimvvIGtZ3ZQh158tdkSnRvr5E_OKgQb4US-yzLzF9KIlZyNZb6T8tLMrBVVY3zD1wnnHa6T7D_AWpBDmZ8tuv-0sF4ybJ_Xd91ceC3-z9j7QLEIoRbjwyIlmGFQLKz-IECmYg/https%3A%2F%2Fwww.rbvi.ucsf.edu%2Fchimera%2Fdocs%2FUsersGuide%2Ffiletypes.html%23volume> ) but Chimera cannot write that format, sorry. You will need to find some other program to convert from mrc to cube, and even so, I don't know if it is the same cube format that the other program wants. I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco > On Feb 21, 2021, at 9:49 PM, Smith Lee <smith_lee123@yahoo.com> wrote: > > Dear All, > > When I use command "mask ones #0" to get the mask for #0 image and use the mask.mrc for further processing by other program, it says the mask.mrc got was not in the "cube volume" format. > > Will you please explain what is the "cube volume" format mrc, and is any way to convert the above mentioned 'non-cube volume" format mask.mrc to "cube volume" format mask.mrc? > > Best wishes, > Smith _______________________________________________ Chimera-users mailing list: Chimera-users@cgl.ucsf.edu Manage subscription: https://secure-web.cisco.com/1niVHy2nRN-giYgBrfC6y6iY1aA4FUL5GMI6ZuVbqAYxX8n... The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Mass General Brigham Compliance HelpLine at http://www.massgeneralbrigham.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.