Dear all,

I am trying to dock atomic coordinate of a protein derived from 3D X-ray crystal (PDB format) into lower resolution (about 20-25 A resolution) EM map (BRIX, XPLOR ASCII or SITUS format) from 2D crystal.

 

1. Does Chimera have this kind of fitting tool (PDB to volume) or I have to fit the coordinate by hand? If chimera can do this task, Which command should I use?
2. Does Chimera have any statistical evaluation for manual fitting?
3. How can you evaluate the % matching of 2 models in Chimera?
 

Best regards,
Puey Ounjai