Dear Experts, This is regarding adding an ALA residue in the C-terminal of a specific protein domain, with CHIMERA. I have a protein-complex with multiple domains and chains. I want to add an ALA residue (101-th position) in the chain D, after the 100th residue which is PRO. I followed the tutorial and have given the following command in command line: *addaa ala,101 :100.d* The following outcome was: Couldn’t find ‘O’ backbone atom of PRO 100.D. Request you to kindly let me know what the possible solution could be, so that I add an ALA residue in the C-terminal of Chain D. Thank you in advance, Regards, Prathit Chatterjee On Mon, Jun 28, 2021 at 4:48 PM Prathit Chatterjee < prathit.biophysics@gmail.com> wrote:
Dear Experts,
This is regarding adding an ALA residue in the C-terminal of a specific protein domain, with CHIMERA.
I have a protein-complex with multiple domains and chains. I want to add an ALA residue (101-th position) in the chain D, after the 100th residue which is PRO.
I followed the tutorial and have given the following command in command line:
*addaa ala,101 :100.d*
The following outcome was: Couldn’t find ‘O’ backbone atom of PRO 100.D.
Request you to kindly let me know what the possible solution could be, so that I add an ALA residue in the C-terminal of Chain D.
Thank you in advance, Regards, Prathit Chatterjee