Thank you for your help! Vani Pande Int PhD Biological sciences IISER Pune 20162013 On Wed, Oct 10, 2018 at 10:24 PM Elaine Meng <meng@cgl.ucsf.edu> wrote:
Hello Vani, Several possibilities if the file is from the PDB and contains symmetry information:
Unit Cell tool (in menu under Tools… Higher-order structure) < http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/unitcell/unitcell....
Multiscale Models tool (in menu under Tools… Higher-order structure) < http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multiscale/framemu...
“sym” command <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/sym.html>
These 3 have overlapping capabilities and it might be that any/all of them would work in your case. See the links for details on what kinds of informatino they use.
You could also go directly to the RCSB PDB website and download the biological assembly instead of just the asymmetric unit.
If the file does not contain symmetry information, that is a different matter. You might have to find another similar structure that does have symmetry information, then in the “sym” command tell the program to use the other protein’s symmetry to make the multimer of your protein.
In case you didn’t know: you can use menu: Help… Search Documentation and look for topics like “symmetry” and it would give the same links as I gave above, plus a few others (Crystal Contacts tool may also be relevant)
I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Oct 10, 2018, at 9:29 AM, Vani Pande < vani.pande@students.iiserpune.ac.in> wrote:
Hello, I wanted to generate symmetry mates for the protein, I am not finding a way to do so in chimera.The protein is a monomer in an asymmetric unit.