Dear Chimera users,

To get Amber prmtop file corresponding to a pdb file using Chimera, I did the following steps:
1] tools -> Amber -> write prmtop
2] In a pop-up window 'save file in chimera', I gave file name, say, aa.prmtop, then clicked save.
3] No charges message appeared, then I clicked 'Assign charges'.
4] In a pop-up window 'Add charge', I selected 'AM1-BCC' in other residues, then clicked ok.
5] In a pop up window 'specify net charge', I selected 'AMI-BCC' in charge method
6] I got 'chimera error' - failure running ANTECHEMBER for residue HIS. Check reply log for details.
7] In reply log, 'Failure running ANTECHEMBER for residue HIS' seen.

Any help in this issue will be a good help for me.

Thank you.
Mahendra Thapa
University of Cincinnati,OH