Hi Tiglath, If you just opened 3r2p one time, you only have one model. Join works on bonding two separate models. Also it contains only chain A (there is no chain B in this structure) and residue 130 in chain A is already bonded to residue 131 in chain A. So I do not understand what you already did to modify the structure(s) before joining, or what you are trying to do now, sorry. You could send a session file if you want. Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Feb 8, 2021, at 12:35 PM, Moradkhan, Tiglath A <tmoradkhan@csus.edu> wrote:
Hi
I'm trying to join two models together using the Build Structure tool but I'm having problems. Here is my process.
• Using the 3R2P crystal structure I select residues 3-130 from chain A and 131-182 from chain B • Then using the following command in Chimera I select the N atom of residue 130 that will form a bond with the C atom of residue 131: sel #0:130@N#1:131@C (#0 is the name of the 3-130 segment and #1 is the name of the 131-182 segment) • Then using the Build Structure tool I select Form C-N peptide bond, but the Apply button doesn't activate. I don't understand what the issue is. Is the command that I've written wrong?
I would be very glad for your help. I can provide the pdb files of the residue segments if necessary. Thanks
Tiglath