Hi Glenn, You can combine the segments in Chimera into one PDB. What I usually do is go to Favorites->Model Panel (which lists all the segments open in your current session), then there is a button on the right labeled 'copy/combine'. You would want the combine functionality. Check the following for the Model Panel window: http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/modelpanel.html <http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/modelpanel.html>Alternatively, there's a command-line version of 'combine' (which I haven't used yet); details here: http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/combine.html Just to add, I would recommend minimizing the structure after combining the segments. This can be done in Chimera with Tools->Structure Editing->Minimize Structure. Also, besides checking the distance between the C and N atoms, you would want to make sure they are in the trans configuration for peptide bonds. In my limited experience, the minimization doesn't always fix peptide bonds that start in the cis configuration. <http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/combine.html>Hope this helps, Tim On Tue, Jun 7, 2011 at 5:30 PM, Glenn Millhauser <glennm@ucsc.edu> wrote:
Hi Folks, I am trying to add a four amino acid segment to the N-terminus of a protein in the pdb. I can generate the four residue stretch and move its C-term close to the N-term of the structured protein. I can then select the C' and N atoms, which are about 1.0 Angstrom apart. At that point, I am stuck. Not sure how to add a bond and then create a proper structure. I've played around with Join Models, but no luck. Without a peptide bond recognized by Chimera, ribbons are discontinuous. Any ideas? Many thanks, glenn
Glenn L. Millhauser Department of Chemistry & Biochemistry UC Santa Cruz Santa Cruz, CA 95064 831 459 2176 voice 831 566 3337 cell 831 459 2935 fax 831 824 4645 google voice
glennm@ucsc.edu
http://chemistry.ucsc.edu/~glennm http://www.chemistry.ucsc.edu/faculty/millhauser.html
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