Dear Haixin, There is a simple way to manipulate part of a protein structure, but it will not make smooth connections between the moved part and the rest of the structure. Take a look at the "Movement Mouse Mode" tool (under Tools... Movement in the menu). You could either choose "Move helix, strand, or turn" if you can accurately grab the helix you want with the mouse, or select your helix and choose "Move selection." I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco On Nov 23, 2009, at 5:21 PM, Haixin Sui wrote:
Tom and Elaine,
Is there a simple way to manipulate part of a protein structure in Chimera? What I would like to do is the following. Let’s say I have a molecular compose 8 helixes. 7 of them fit in the cryo-EM map perfectly but one short helix is off probably owing to conformational change. I would like to move the short helix into the density map manually. The difficult part is how to make a smooth transition for loop regions at both ends of the helix. Would you please give me some advice on this?
Thanks! Haixin