On Dec 3, 2021, at 6:44 AM, SANDOVAL, KENNETH <K.SANDOVAL1@nuigalway.ie> wrote:

Hello, I have a predicted secondary structure of an mRNA molecule. I am interested in trying to visualize this with UCSF Chimera. But I'm unsure what file format the program accepts for this? Does it need some sort of mRNA equivalent of a .pdb file? Thank you, Kenneth

Hello Kenneth, The recommended address for Chimera questions is chimera-users@cgl.ucsf.edu CC'd here. By secondary structure I'm guessing that you mean the base-pairing pattern, and not actually 3D atomic coordinates. For Chimera you would generally need 3D atomic coordinates, most commonly in PDB format. If that is really what you have (base-pairing information only), then here are two ideas for building a 3D structure in Chimera using that information: (1) The RNArtist plugin to Chimera - quite extensive, has a lot of nice features, needs to be downloaded separately: <https://github.com/fjossinet/RNArtist> <https://www.rbvi.ucsf.edu/chimera/plugins/plugins.html> (2) The "rna" command in Chimera can also build models but much more roughly. See "rna model": <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/rna.html> <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/rna.html#model> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco