How to show local agreement between cryo-EM map and model
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Dear developers and users, Is it possible to generate/color a cryo-EM map to show local agreement between the map and the model generated using the same map? If I am not wrong, the following figure had accomplished the job but I don't understand how to generate a similar figure for my project: http://d2ni3bh4dzb2ig.cloudfront.net/content/embojnl/28/6/755/DC7/embed/inli... Best regards, Tofayel NTU Singapore
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HI Tofayel, The Chimera command to measure the local correlation between two maps using a sliding window is “vop localCorrelation”. Here is a past mailing list post about its use http://www.cgl.ucsf.edu/pipermail/chimera-users/2012-October/008076.html <http://www.cgl.ucsf.edu/pipermail/chimera-users/2012-October/008076.html> Basically you make a map from your atomic model using the Chimera molmap command then correlation over a small box centered at each point of the map grid and color using that correlation value. Here is the documentation on vop localCorrelation https://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#localCorrela... Tom
On Apr 16, 2017, at 6:36 AM, #AHMED TOFAYEL# wrote:
Dear developers and users,
Is it possible to generate/color a cryo-EM map to show local agreement between the map and the model generated using the same map? If I am not wrong, the following figure had accomplished the job but I don't understand how to generate a similar figure for my project:
http://d2ni3bh4dzb2ig.cloudfront.net/content/embojnl/28/6/755/DC7/embed/inli... <http://d2ni3bh4dzb2ig.cloudfront.net/content/embojnl/28/6/755/DC7/embed/inline-supplementary-material-7.jpg?download=true>
Best regards, Tofayel NTU Singapore
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participants (2)
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#AHMED TOFAYEL#
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Tom Goddard