copy transformation between volumes
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Dear community, I need your advice on the following problem. I align map #1 to the reference volume using fit command, and then need to confer this transformation to map #2. This could be done by using matrixcopy command. However in this particular case #1 has twice larger voxel size than #2. It is understod, that since transformation matrix describes shifts in non-scaled pixels, I can not use matrixcopy command. Is it correct to scale translations (column 4) in the fit matrix for #1 and apply it to #2 with matrixset command? Thank you and best regards, Oleksiy Dr Oleksiy Kovtun Research Fellow Briggs group MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH UK Tel: + 44 1223 267551 Email: okovtun@mrc-lmb.cam.ac.uk
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Hi Oleksiy, The "matrixcopy #1 #2" command should do what you want. It does not matter that the voxel size is different. The matrix being copied is one that transforms the model physical coordinate system (usually in Angstroms) to the world coordinate system (also in Angstroms). The voxel size is how grid indices are converted to physical units (Angstroms) and is not a factor for aligning the two maps with matrixcopy. Tom
On Aug 29, 2019, at 1:35 PM, Oleksiy Kovtun <okovtun@mrc-lmb.cam.ac.uk> wrote:
Dear community, I need your advice on the following problem. I align map #1 to the reference volume using fit command, and then need to confer this transformation to map #2. This could be done by using matrixcopy command. However in this particular case #1 has twice larger voxel size than #2. It is understod, that since transformation matrix describes shifts in non-scaled pixels, I can not use matrixcopy command. Is it correct to scale translations (column 4) in the fit matrix for #1 and apply it to #2 with matrixset command?
Thank you and best regards,
Oleksiy
Dr Oleksiy Kovtun Research Fellow Briggs group MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH UK
Tel: + 44 1223 267551 Email: okovtun@mrc-lmb.cam.ac.uk <mailto:okovtun@mrc-lmb.cam.ac.uk>
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participants (2)
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Oleksiy Kovtun
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Tom Goddard