Hi Svetla, Chimera is correctly handling your 880x880x880 map. But by default Chimera displays only a single plane of very large maps, because the most common case of this is with tomograms where a single plane display is usually what you want. When it does this the Volume Viewer dialog shows a "Planes" slider in the dialog, and below that is a button "All" that will show all planes. If you want Chimera to always show the full map, use Volume Viewer menu entry Features / Data Display Options, and click off "Show plane when data larger than N Mvoxels", and then use Features / Save Default Dialog Settings so it will use that setting in all future uses of Chimera. Tom On Jun 5, 2013, at 9:54 AM, "Stoilova-McPhie, Svetla" wrote:

Dear Tom,

Are there any limitation for the size of 3D map/volume the Volume viewer can visualize?

We tried a 880x880x880 voxels map generated with spider and it comes out as a 2D map: 880x880x1. WE triple checked and the volume file dimensions are indeed 880x880x880 and can be visualized as a 3D volume in the EMAN2 viewer.

Please advise.

Best regards,

Svetla Stoilova-McPhie, PhD Assistant Professor, Department of Neuroscience and Cell Biology Scientist, Sealy Centre for Structural Biology and Molecular Biophysics University of Texas Medical Branch at Galveston 301 University Boulevard, Galveston, Texas 77555-0620