Hi all, Can I combine two mrc maps into one? And the two maps are with different pixel size. One is 1.48 A, the other is 2.33 A. I want to combine the two into one with pixel size of 2.33 A. I tried segmentation, and made two maps. But I failed to combine them. Can anybody help me for that? Thanks. Sincerely yours, Jian
Hi Jian, See the “vop” command: there are options to add, subtract, multiply, and do several other things to maps. There is a sub-option “onGrid” to specify the grid of the output map. <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco On Oct 24, 2014, at 7:31 AM, Jian Guan <jug25@psu.edu> wrote:
Hi all, Can I combine two mrc maps into one? And the two maps are with different pixel size. One is 1.48 A, the other is 2.33 A. I want to combine the two into one with pixel size of 2.33 A. I tried segmentation, and made two maps. But I failed to combine them. Can anybody help me for that? Thanks. Sincerely yours, Jian
Hi Elaine, Got it. Thank you so much for your help. You have great weekend. Jian -----Original Message----- From: Elaine Meng [mailto:meng@cgl.ucsf.edu] Sent: Friday, October 24, 2014 1:07 PM To: Jian Guan Cc: chimera-users@cgl.ucsf.edu Subject: Re: [Chimera-users] Combine maps Hi Jian, See the "vop" command: there are options to add, subtract, multiply, and do several other things to maps. There is a sub-option "onGrid" to specify the grid of the output map. <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco On Oct 24, 2014, at 7:31 AM, Jian Guan <jug25@psu.edu> wrote:
Hi all, Can I combine two mrc maps into one? And the two maps are with different pixel size. One is 1.48 A, the other is 2.33 A. I want to combine the two into one with pixel size of 2.33 A. I tried segmentation, and made two maps. But I failed to combine them. Can anybody help me for that? Thanks. Sincerely yours, Jian
Hi Jian, You can add your two maps with the volume operation command vop add #1,2 This will create a new map with grid points that align with map #1, with map #2 interpolated (trilinear interpolation) at those grid points. The bounds of the new map will extend beyond the bounds of #1 if that is needed to cover map #2. Here is documentation http://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/midas/vop.html#add If density in your two maps partially overlap each other then adding them together will create extra high density in the overlap region which is probably not what you want. How to blend them together in the overlap region is a difficult problem that Chimera does not have any capability to handle. Tom On Oct 24, 2014, at 7:31 AM, Jian Guan wrote:
Hi all, Can I combine two mrc maps into one? And the two maps are with different pixel size. One is 1.48 A, the other is 2.33 A. I want to combine the two into one with pixel size of 2.33 A. I tried segmentation, and made two maps. But I failed to combine them. Can anybody help me for that? Thanks. Sincerely yours, Jian
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participants (3)
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Elaine Meng
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Jian Guan
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Tom Goddard