Hi Chinmaya, If you are using the Chimera command line, you want Chimera commands, not python. Here is how to use the "open" command, <http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/open.html> see the part about local files, <http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/open.html#local> However, based on our earlier correspondence, I somewhat doubt you have a PNG stack. Just having multiple PNG files does not mean that they are a stack. An image stack is generally produced by some instrument (say a microscope) and consists of 2D images that when opened together and slightly displaced from one another along the third dimension, create a single 3D map or object. <http://www.cgl.ucsf.edu/chimera/docs/ContributedSoftware/volumeviewer/images...> Elaine On Jun 16, 2011, at 4:59 PM, chinmaya joshi wrote:

Can you help me how I can open a stack of .png images using a command line in chimera. I will have a set of images and I want to open the stack in chimera using the command line. Is there any python script for this?

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