Hi Agne, If you were using the steps on this page: <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/surfnorm.html> ... then the "relSESA" values are the surface area of the specific residue normalized (divided) by the surface area of that same type of residue in the middle of a Gly-X-Gly tripeptide. The GXG tripeptide is just meant to represent a fully exposed state of that type of residue. For example, 1.0 is totally exposed (same as in GXG tripeptide), 0.2 is 20% exposed compared to the GXG tripeptide, 0.0 is fully buried, etc. I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On May 16, 2020, at 12:08 AM, <agnek54@gmail.com> <agnek54@gmail.com> wrote:

Hello,

I would like to consult on relative exposure of amino acids calculations interpretation. I performed the steps for relative amino acid exposure calculation for my structure, however I am a bit lost on how to interpret the results. Since the parameter used for calculation is solvent excluded area, I am a little bit confused about relSESA attribute values meaning: does the highest value for relSESA attribute show that residue is highly exposed, or highly buried in the structure?

Sincerely, Agne