Hi Masako, Chimera does not read IMOD files but perhaps I can add a file reader for you. Have you already defined a path in IMOD along the structure you want to measure? Or have you just segmented out the structures. If you have segmentations but no explicitly defined 1 dimensional path then it is not clear how to define the length. Is the IMOD format you are using the following? http://bio3d.colorado.edu/imod/doc/binspec.html Could you send me an example file? Tom

Date: Mon, 11 Sep 2006 17:41:20 -0700 From: Masako Terada <mterada@ncmir.ucsd.edu> To: Thomas Goddard <goddard@cgl.ucsf.edu> Subject: Re: [Chimera-users] Can I use "Chimera" to measure length of a VRML file?

Hi. Thank you for your e-mail. I have tried using volume path tracer as you instructed. Even though it looks very promising, it is time-consuming since we have 90 different objects to measure. So I would like to try your another suggestion, importing trace file into Chimera and measure lengths. Are you familiar with IMOD segmentation file format? I tried to import this IMOD file into Chimera, but unsuccessful, so I am guessing that I have to convert it to something else... Any more suggestion?

Masako