Dear colleagues,
I have similar cryo-em maps that have also some differences. I want to compare them.
Is there a tool in chimera that can compare the cryo-em maps?
Thank you!
Sincerely, Dmitry
Hi Dmitry, Depends what you mean by “compare.”
Some thoughts are:
(A) subtract one map from the other to see the differences: command “vop subtract” http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#subtract
(B) show the same isosurface level for both, Measure Volume and Area (under Tools… Volume Data) or the corresponding commands “measure volume” “measure area”
http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/measurevol/measurevol.html http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/measure.html#volume http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/measure.html#area
(C) morph between their isosurfaces with Morph Map tool or command “vop morph" http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/morphmap/morphmap.html http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#morph
This tutorial has an example of morphing between two EM maps (about halfway down the page): http://www.rbvi.ucsf.edu/chimera/data/tutorials/eman07/chimera-eman-2007.html
I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Feb 28, 2020, at 1:58 AM, Dmitry Semchonok Semchonok@gmail.com wrote:
Dear colleagues, I have similar cryo-em maps that have also some differences. I want to compare them. Is there a tool in chimera that can compare the cryo-em maps? Thank you! Sincerely, Dmitry
participants (2)
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Dmitry Semchonok
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Elaine Meng