Hi Yanhe, (1) when you want commands to execute simultaneously, just put them together with semicolons and no wait between them. E.g. something like: volume #0 planes z,0,50,1; turn y 1 50; wait 50 Command file rules and tips: <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/indexcommand.html#cmdfile> (2) you cannot merge 3 volumes into one volume with each one keeping its own different threshold, sorry. You can combine maps in several different ways (add, subtract, maximum, minimum, etc. as with "vop" command) but the merged result is one map. One map can have multiple thresholds but they would all apply to the whole map. <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On Jun 1, 2021, at 10:50 AM, Yanhe Zhao <yanhezhao1990@gmail.com> wrote:

Good afternoon Elaine and users,

I have two questions as to Chimera using as below:

1, how can I plane through and rotate volume at same time? For example how to add rotate options to command "volume #2 planes x,0,42,1; wait 43;"?

2, How I can merge volumes (for example 3 volumes) with their own threshold was kept?

Thanks and best, Yanhe