Hi Tamas, Chimera Volume Viewer is reporting the range of data values. Probably it is showing you a subsampled version of the data (e.g. “step 2”) and then it only shows the min and max of the subsampled values. Change step (above the histogram) to 1 to load the full resolution data. Chimera automatically chooses the step to open the data fast. This is a setting in Volume Viewer menu Features / Data Display Options called “Adjust step to show at most N voxels”. Tom

On May 22, 2018, at 2:23 PM, Tamas Hegedus <tamas@hegelab.org> wrote:

Hi,

I have a .map file.

If I open it in chimera, volume viewer indicates a range (-0.133 - 0.235 Level: 0.018 (in a textbox)), which range is different from the min and max values of data in the map file (read by python mrcfile).

The chimera's range is narrower.

- why is that?

- I can imagine that chimera apply some cutoff; if this is the case, how can I display the full range of the .map file?

Thanks, Tamas

-- Tamas Hegedus, PhD Senior Research Fellow MTA-SE Molecular Biophysics Research Group Hungarian Academy of Sciences | phone: (36) 1-459 1500/60233 Semmelweis University | fax: (36) 1-266 6656 Tuzolto utca 37-47 | mailto:tamas@hegelab.org Budapest, 1094, Hungary | http://www.hegelab.org

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