Numbering the amino acid sequence

A referee is asking me to number the amino acid sequence involved in docking a ligand with a protein. Not satisfied by having a side table with a guideline. Is it possible to add that numbering in a least-intruding way? The figure exported in standard graphic formats from Chimera is already extremely crowded. Therefore, is any possibility of adding the numbering outside the protein with some kind of pointers? I can't do with colors alone: I already used different colors for different helices. Thanks francesco pietra ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ

Outside of Chimera, there are several ways to do this in image editing programs (Gimp, Photoshop, etc.). Use layers and: 1) draw arrows; or 2) place the number on top of a box just big enough to fit the text (opaque to semi-transparent); or 3) use effects on the text, such as adding an outer glow effect in Photoshop, which adds a halo around the text so that its outline stands out from the image, making it readable. I would advise not going crazy with colors, this just adds to the complexity of the image. Cheers, Jeff On Apr 27, 2008, at 10:49 AM, Francesco Pietra wrote:
A referee is asking me to number the amino acid sequence involved in docking a ligand with a protein. Not satisfied by having a side table with a guideline.
Is it possible to add that numbering in a least-intruding way? The figure exported in standard graphic formats from Chimera is already extremely crowded. Therefore, is any possibility of adding the numbering outside the protein with some kind of pointers? I can't do with colors alone: I already used different colors for different helices.
Thanks francesco pietra
______________________________________________________________________ ______________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http:// mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Chimera-users mailing list Chimera-users@cgl.ucsf.edu http://www.cgl.ucsf.edu/mailman/listinfo/chimera-users

Also, the 2D Labels tool in Chimera (under Utilities) can place labels at arbitrary positions on the screen. However it doesn't have the capability of drawing arrows yet, so if that is crucial to the understandability of the image you will have to make use of Jeff's excellent suggestions instead. --Eric On Apr 28, 2008, at 9:07 AM, Jeff Speir wrote:
Outside of Chimera, there are several ways to do this in image editing programs (Gimp, Photoshop, etc.). Use layers and: 1) draw arrows; or 2) place the number on top of a box just big enough to fit the text (opaque to semi-transparent); or 3) use effects on the text, such as adding an outer glow effect in Photoshop, which adds a halo around the text so that its outline stands out from the image, making it readable.
I would advise not going crazy with colors, this just adds to the complexity of the image.
Cheers,
Jeff
On Apr 27, 2008, at 10:49 AM, Francesco Pietra wrote:
A referee is asking me to number the amino acid sequence involved in docking a ligand with a protein. Not satisfied by having a side table with a guideline.
Is it possible to add that numbering in a least-intruding way? The figure exported in standard graphic formats from Chimera is already extremely crowded. Therefore, is any possibility of adding the numbering outside the protein with some kind of pointers? I can't do with colors alone: I already used different colors for different helices.
Thanks francesco pietra
_____________________________________________________________________ _ ______________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http:// mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Chimera-users mailing list Chimera-users@cgl.ucsf.edu http://www.cgl.ucsf.edu/mailman/listinfo/chimera-users
_______________________________________________ Chimera-users mailing list Chimera-users@cgl.ucsf.edu http://www.cgl.ucsf.edu/mailman/listinfo/chimera-users
participants (3)
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Eric Pettersen
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Francesco Pietra
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Jeff Speir