Hello, Since this is a ChimeraX question it should go to chimerax-users@cgl.ucsf.edu instead of chimera-users@cgl.ucsf.edu (we don't want to confuse the Chimera users). But since the answer is short I will try to give it now: sorry, there is no ChimeraX option to control which template(s) the alphafold calculation uses. There is an option "Use PDB templates when predicting structures" but that is only to control whether it uses any templates or not. Default is off, do not use any templates. <https://rbvi.ucsf.edu/chimerax/docs/user/tools/alphafold.html#options> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On Jul 13, 2023, at 3:43 AM, Enrico Martinez via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:

Dear Chimera-X users!

In parallel to the modeller, I am trying to use the Alpha fold interface with the aim to full model starting from the sequence using template.

The problem is that I can not define manually which template I would like to use for each prediction so the interphase takes its automatically based on the input sequence and the final prediction is not very precise. Would it be possible rather to specify a specific pdb ID additionally to a given sequence ?

Many thanks in advance