If you wanted to separate out say the high resolution part of your map you could mask your EM map using a contour surface of your local resolution map. Suppose your EM map is #1 and your local resolution map is #2 then I think ChimeraX commands like volume #2 level 3.0 volume mask #1 surface #2 invertMask true would separate the part that has resolution less than or equal to 3 Angstroms. The "invertMask true" option is because you want to keep the parts at values lower than 3 while by default it would keep the parts higher than 3. Here is ChimeraX "volume mask" documentation https://www.cgl.ucsf.edu/chimerax/docs/user/commands/volume.html#mask There is a mask command in Chimera that can probably do the same thing. Tom

On Oct 24, 2024, at 8:43 AM, Elaine Meng via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:

Hi JPD, Color Zone will split by the colors applied by Color Zone, which are based on distances to atom or markers. It will not split by other coloring (such as by local resolution) which is just on the surface and continuously varying, rather than in zones with exactly the same discrete value.

I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Resource for Biocomputing, Visualization, and Informatics Department of Pharmaceutical Chemistry University of California, San Francisco

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On Oct 23, 2024, at 8:59 AM, jp d via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:

hi, i have a local resolution map from cryosparc i would like to split it by color (resolution) it seems like Tools -> Color zone may do this ?

i can display the local resolution map and it colors correctly, but when i try to split it with color zone, it say the map has no color zone. any pointers or suggestion would be appreciated.

thanks jpd

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