Dear Ernest, Sorry, you will need to use your own scientific judgment as to how to interpret such differences. Maybe you can find some colleagues or laboratory members with which you can discuss the differences and make hypotheses about whether any of the differences have functional or stability implications. There may not be anything you can conclude from the measurements alone -- you may need to combine them with experimental findings for the mutant, as well as anything that is already known about how that specific protein works. Regards, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On Aug 8, 2022, at 6:12 AM, Ernest Matambo via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:

Dear Sir/Madam, I am using Chimera 1.16 to compare two protein structures: one with mutations and a reference. I would like to calculate residue-residue distance of the substitute amino acids to the reference. I have used chimera to come up with a graph of the difference but I am struggling to interpret it. Any help will be greatly appreciated. Regards, Ernest