Hi Anaswara, Chimera does not have a tool specifically to identify the membrane boundaries. As a clue to figuring it out yourself if you have an atomic structure of the protein, you could display amino acid hydrophobicity on the surface, for example as in this tutorial: <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/tutorials/surfprop.html> ...but it would not guess a boundary for you. Outside of Chimera: Thanks to Tamas for his suggestion, for cryoEM maps! Another thing that might be useful for atomic structures is the Orientations of Proteins in Membranes database: <https://opm.phar.umich.edu/> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On Mar 12, 2019, at 9:06 AM, Tamas Hegedus <tamas@hegelab.org> wrote:

Hi, Could you give more specification what a where do you want? We just resubmitted our manuscript on determination of membrane boundaries from cryo-EM densities. You can try our web app:

http://memblob.hegelab.org

Please provide feedback on bugs. Thanks, Tamas

On 3/12/19 3:54 PM, Anaswara Ashok wrote:

...

Hi I would like to know if we could add membrane boundaries of proteins with chimera? -- Regards Anaswara Ashok

-- Tamas Hegedus, PhD Senior Research Fellow Department of Biophysics and Radiation Biology Semmelweis University | phone: (36) 1-459 1500/60233 Tuzolto utca 37-47 | mailto:tamas@hegelab.org Budapest, 1094, Hungary | http://www.hegelab.org