Hi Dominique, Segger menu Regions / Subtract Selected From Map operates on the map listed in the "Segment Map" menu at the top of the Segger dialog. So when I segment one map, then change the Segment Map menu to another map and use that menu entry it subtracts from that new map that was not the one the segmentation was calculated from. The new map does need to be the same size in grid points and have the same alignment. Tom

On Feb 20, 2020, at 10:18 AM, Dominique Gutierrez <dgutierrez@gradcenter.cuny.edu> wrote:

Hello,

Can I use the "subtract selected segment from map" in Segger tool to subtract the selected from a specific map and not just the map used for segmentation. In my case the segmented map is from a vop subtracted intermediate used to facilitate segmentation but I would like to quickly subtract several segments from the original map. Or is the only way to do that with vop subtract?

Best, Dominique _______________________________________________ Chimera-users mailing list: Chimera-users@cgl.ucsf.edu <mailto:Chimera-users@cgl.ucsf.edu> Manage subscription: http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users <http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users>