Hi Daniel, You can add or subtract maps to make a new map using the "vop" command: <http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html> That command has various other filtering and scaling options that may also be of interest. You can save the new map to a file using the Volume Viewer dialog (its File menu) or the "volume" command. I hope this helps, Elaine ---------- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco On Aug 1, 2012, at 6:58 PM, Daniel Murin wrote:

Hi, I was wondering if it is possible to combine two mrc volumes into a single volume and save that as a new mrc file? I haven't really been able to find anything and I suspect that this may not be possible with Chimera. Thank you for your help, Daniel

Hi Daniel, Yes, just add the two map with the volume operation command vop add #1,2 This will extend the grid of map #1 to cover map #2 then interpolate map #2 on that grid and add it. You may also want to scale the values of one map so that the two maps have comparable density ranges using the "scaleFactors" option. http://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/midas/vop.html#add Tom

Hi,

I was wondering if it is possible to combine two mrc volumes into a single volume and save that as a new mrc file? I haven't really been able to find anything and I suspect that this may not be possible with Chimera.

Thank you for your help,

Daniel

Charles Daniel Murin Graduate Student - Ward/Ollmann-Saphire Labs Department of Molecular Biology Kellogg School of Science and Technology The Scripps Research Institute 10550 North Torrey Pines Road, CB 227 La Jolla, CA 92037