Hi Sudheer, If you have a monomer PDB model fit into the map you could display a zone around it http://www.cgl.ucsf.edu/chimera/tutorials/volumetour/volumetour.html#zone and save that part of the map to a file with volume dialog File / Save Map As.... Or you could use volume eraser to erase everything but one monomer http://www.cgl.ucsf.edu/chimera/tutorials/volumetour/volumetour.html#handera... Or you could trace loops plane by plane, join them to form a surface using volume tracer http://www.cgl.ucsf.edu/chimera/docs/ContributedSoftware/volumepathtracer/fr... then use the mask command to extract the region within the surface http://www.cgl.ucsf.edu/chimera/tutorials/volumetour/volumetour.html#mask Just knowing the 7-fold symmetry doesn't tell you the boundaries of the monomers. There is currently no tool to simply extract a 1/7 wedge. Tom skmolugu@miners.utep.edu wrote:

Hi, I have a CryoEM density map with 14 subunits with seven fold symmetry. Is there a way in chimera to separate only one subunit from the entire map. thanks, sudheer _______________________________________________ Chimera-users mailing list Chimera-users@cgl.ucsf.edu http://www.cgl.ucsf.edu/mailman/listinfo/chimera-users