Dear Ashok, If the NMR structure has multiple conformations, you first have to decide which one you want and close the others. About that, see this post from yesterday: <http://plato.cgl.ucsf.edu/pipermail/chimera-users/2016-April/012204.html> Next, delete hydrogens, for example, command: delete H You could also do it with the menu. To start learning Chimera, you could try the “getting started” tutorials: <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/frametut.html> Next, show electrostatic potential coloring on the surface. There is already a step-by-step tutorial for that, and it includes two different ways (Coulombic and Poisson-Boltzmann): <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/tutorials/surfprop.html> Best, Elaine ---------- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco

On Apr 26, 2016, at 5:13 AM, ashok rout <ashok9869@gmail.com> wrote:

Hi, I have NMR structure of a protein. I want to plot electrostatic surface charge distribution. Can you please write me step by step commands how I can do it. I want to remove hydrogens first and then want to plot electrostatic surface charge distribution. Ashok

-- thanks & regards, Ashok