Hello Andres, The error message says that there are nonstandard hydrogen names. If I restore your session, then just delete the hydrogens, command: delete H ...and then minimize (minimization prep will add them back), it works for me. By the way, you can easily mutate residues in Chimera, see command “swapaa” <http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/swapaa.html> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco

On Feb 9, 2018, at 5:50 PM, Andres Benitez <andresbenitez@gmail.com> wrote:

Hello,

I am trying to use minimize to resolve side chain positions which were mutated using pymol. The file is saved as a pdb, but I am getting errors when I try to use the minimize function. I am wondering if the error is because the structure has some loops removed, or if there is something else going on. I've attached the chimera session file and a text file with the errors I am getting.

Thanks,

Andres Benitez <error_minimize.txt><DRD2_mutated.py>