Enquiry: Match Maker

Hi, I have a question regarding structural alignments with Match Maker. I would like to know if there is any way to obtain a list of all possible alignment solutions (or a subset) tested by Match Maker, instead of only the "best" one, so that I can further assess the different options manually. Specifically I am trying to find an homologous substructure/motif from my template structure within a query structure. The pattern to be searched is rather small as compared to the whole protein and it can be aligned in multiple positions. I would like to assess each of the possibilities more carefully based on the biological knowledge of the protein. Thank you in advance. Best wishes, Anna Hernandez

Hi Anna, My first impression is that MatchMaker is not the appropriate tool for such a study. You may instead wish to use one of the many "3D motif"-related software tools. There may be dozens of such resources, but to name a few: LabelHash server <http://labelhash.kavrakilab.org/> PINTS server <http://www.russelllab.org/cgi-bin/tools/pints.pl> funClust server <http://pdbfun.uniroma2.it/funclust/index2.py> pdbFun server <http://pdbfun.uniroma2.it/> I hope this helps, Elaine ---------- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco On May 6, 2014, at 2:59 AM, Anna Hernandez <annahernandezduran@gmail.com> wrote:
Hi,
I have a question regarding structural alignments with Match Maker. I would like to know if there is any way to obtain a list of all possible alignment solutions (or a subset) tested by Match Maker, instead of only the "best" one, so that I can further assess the different options manually.
Specifically I am trying to find an homologous substructure/motif from my template structure within a query structure. The pattern to be searched is rather small as compared to the whole protein and it can be aligned in multiple positions. I would like to assess each of the possibilities more carefully based on the biological knowledge of the protein.
Thank you in advance.
Best wishes,
Anna Hernandez
participants (2)
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Anna Hernandez
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Elaine Meng