Hi Gian, To use "Area/Volume from Web" you need to get a newer version of Chimera. That Web server changed and we had to change Chimera accordingly. However, you do not need to use this tool to calculate areaSAS and areaSES. You only need to display molecular surface in Chimera and these are automatically computed, as described here: http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/ representation.html#surfaces Totals are reported in the Reply Log, and values per atom and per residue are assigned as the attributes areaSAS and areaSES. This feature is also newer than the production release from last fall, so I recommend you get a daily build: http://www.cgl.ucsf.edu/chimera/alpha-downloads.html It sounds like you were looking at documentation newer than the version you had installed. If you use the documentation from the Help menu, it should match what you have installed. However, in this case it is good that you found out about the newer features... now you just need the version that includes them! I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. meng@cgl.ucsf.edu UCSF Computer Graphics Lab and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco http://www.cgl.ucsf.edu/home/meng/index.html On May 1, 2008, at 5:25 PM, De Nicola, Gian wrote:

To whom it may concern I would like to calculate the areaSAS and areasSES using Chimera.This is what I do Load the structure tools Surface Binding Analysis area/volume from web I choose the type of surface I want. Click apply then Ok As an output I get " unexpected output from struc tools server" . What am I doing wrong? Regards Gian