Hi Stefan, Earlier this year the RCSB stopped allowing HTTP access on the URL that Chimera uses to fetch information about the blast hits (only allowing HTTPS access). So the Chimera production release has the problem you've seen. The daily build has the updated URL, so if you install the daily build you will be able to run blast successfully. Sorry for the hassle you went through. --Eric Eric Pettersen UCSF Computer Graphics Lab

On Jul 14, 2021, at 2:50 AM, Stefan Dobrev via Chimera-users <chimera-users@cgl.ucsf.edu> wrote:

Hello!

I'm trying to run a homology model on a protein sequence using BLAST, but my Blast: query window shows up empty. At first I got this error in the reply log:

File "C:\Program Files\Chimera 1.15\share\blastpdb\pdbinfo.py", line 151, in fetch_pdb_info import replyobj ImportError: No module named replyobj

I solved it by changing the code to "from python import replyobj". Now I can actually see what the error is. A few more functions are called until we get to this:

File "C:\Program Files\Chimera 1.15\bin\lib\socket.py", line 480, in readline data = self._sock.recv(self._rbufsize) error: [Errno 10054] An existing connection was forcibly closed by the remote host

I've allowed chimera through the firewall and I have a modeller license key, I believe I've entered it somewhere in Chimera at some point. Any ideas what might be causing this?

Thanks for any advice, Stefan Dobrev _______________________________________________ Chimera-users mailing list: Chimera-users@cgl.ucsf.edu Manage subscription: https://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users