Hi Elaine! Thank you the Vop sesample is exactly what I was looking for! Great! Sara

Hi Sara, The map formats don't include rotation information, so you would need to resample the rotated map on the grid of the other map. This interpolates and makes a new map model.

Please see here for details on how to save a map after fitting: <http://www.cgl.ucsf.edu/chimera/docs/UsersGuide/savemodel.html#afterfitting>

However, Chimera does not write the spider format. You would need to save in MRC, NetCDF, Chimera map, or BRIX format. I hope this helps, Elaine ---------- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco

On Jun 15, 2012, at 7:16 AM, Sara Sandin wrote:

...

Hi, Thank you for a great software :)

I have one problem. I have two em density maps in spider format.

After I have used the fit in map option and one density has been rotated and shifted, how do I save a new spider file with the applied rotation and translation?

Thank you, Sara

-- Dr Sara Sandin Division of Structural Studies MRC Laboratory of Molecular Biology Hills Road Cambridge CB2 0QH UK ssandin@mrc-lmb.cam.ac.uk