
Hi, How can i save some region of an EM data in Chimera. I can corp the region of my interest in EM map using chimera but I am unable to save the data in mrc format. How can i save this region of EM data in mrc file. Thanks for your help. Best, surnedra

Hi Surendra, Chimera is not able to write MRC files. Perhaps you know of a separate program that can extract a region of an MRC file. You can get the bounds of the cropped region from Chimera. Turn on "Show bounds and step in region panel" in the volume viewer / options panel. Then the volume viewer region panel will show the matrix indices (zero based) corresponding to your cropped region. I do not know of an easy to use program that extracts MRC subregions. The EMAN package proc3d program can probably do it. The SPIDER package can probably also do it. These are packages for processing electron microscope data that can be found on the web. Tom
participants (2)
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Negi, Surendra S.
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Thomas Goddard