Hi Tom, The morph tool just linearly interpolates between the grid points in the two maps. So it doesn't use a new alignment of the maps that you did before morphing. To make that work you should interpolate one of the maps on the grid of the other map after you've made the alignment. Then you can morph between the two maps which are now defined on the same grid. To do that interpolation use the vop (volume operation command): vop resample #1 ongrid #0 This command makes a new copy of map #0 interpolating on the grid of map #1. Another issue that may arise is that the map density values are scaled differently. You can apply a scale factor in the Morph Map options panel to correct that. Here's a video tutorial that demonstrates the alignment, interpolation, and scale factor http://www.cgl.ucsf.edu/chimera/videodoc/MorphAlign Tom

Hi,

I have two 3D-EM volumes that I wish to use the morph option for. I can align the two maps in chimera by hand so that they sit well together but the problem is I want to be able to create a morph video from the maps new positions. When I use the morph option it only allows me to create a morph from the original set starting positions of each volume. Is there a way to get around this? I would be very thankful for any help or guidance.

Tom _______________________________________________ Chimera-users mailing list Chimera-users@cgl.ucsf.edu http://plato.cgl.ucsf.edu/mailman/listinfo/chimera-users