Dear Elaine, It is diffrent from the previou post. In the previous shift, the map and pdb did not fit (caused by an accident) in Chimera, and thus they did not fit in Coot. In my situation, the PDB and map fit in Chimera, but did not fit in Coot. Thus can you think out someway to have them fit in Coot? Smith At 2015-03-19 23:35:15, "Elaine Meng" <meng@cgl.ucsf.edu> wrote:

Dear Smith, It may be the same issue as discussed in this previous post -- if so, it is a Coot problem, but a workaround is described:

<http://plato.cgl.ucsf.edu/pipermail/chimera-users/2015-February/010736.html>

I hope this helps, Elaine ---------- Elaine C. Meng, Ph.D. UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab Department of Pharmaceutical Chemistry University of California, San Francisco

On Mar 18, 2015, at 11:10 PM, "Smith Liu" <smith_liu123@163.com> wrote:

...

Dear All,

I have fit a PDB file with a MRC by the Chimera. I saved both the fitted PDB file and the fitted MRC file by coot. When I open the fitted PDB file and the fitted MRC file by Chimera at the same time, they still fit perfectly. However when I open the Chimera fitted PDB file and the Chimera fitted MRC file by coot, the protein backbone (and the sidechains) does not fit the MRC file anymore.

Will you please tell me what should I do in order to have the Chimera fitted PDB file and the Chimera fitted MRC file still fit in Coot?

I am looking forward to getting your reply.

Smith