Dear Tom, Dear Elaine Thanks for your thorough explanation. I am working with 42 structures, all produced by the af3 server. The one I sent was an MRE. @Tom: To sum up, 1. Those outputs summarise the interface properties in terms of contacts, and the most synthetic indicator of interface confidence/stability [1] is confident pairs. When contextualising this indicator, one should remember that since proximity can be many-to-one and confidence is based on PAE, the number of confident pairs cannot be related to the sum of residues involved from the two chains. Also, when using AF3 models, one should be sure not to have nonstandard residues and/or ligands and rely on the CSV. [1] Maybe stability is too much... P.S.: Regarding netiquette, should I also reply to your address or the mailing list? -- Bruno Hay Mele, PhD 2D-20, Biology Dept., University of Naples Federico II https://github.com/bhym/ | +39 081 67 9118