Hi Thibault,
I haven't been able to come up with a combination of ChimeraX settings to make an image like that.  

Starting from flat (first image attached), as soon as I turn on multishadows (light multishadow 128), it looks 3D, not flat (second image attached).  

So I also tried instead using depth-cueing (light depthcuestart 0.1 depthcueend .75 depthcuecolor black depthcue true  -> third image attached), but that doesn't achieve a similar appearance either. 

Maybe someone else can come up with how to make an image similar to yours, but as far as I can tell, it may not be possible.
Best,
Elaine
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Elaine C. Meng, Ph.D.
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

On May 26, 2022, at 10:44 AM, Thibault TUBIANA via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:

Dear Chimera X community and developpers :) 

I would like to create a flat representation for a protein but with ambiant occlusion, a little bit like on the example joined (made with https://3dproteinimaging.com).
Is it something that it is possible with ChimeraX ? I've been playing with the shaddows but the "flat effect" is disappearing then :/ 

Thank you :) 
Thibault.