Hello, with Avogadro I built and optimized simple tri-peptide which I read as pdb into ChimeraX, see the attachments. When I wanted to display coulombic potential, I got the following error message below. Looks like Avogadro's pdb protocol does not fit for ChimeraX...although all atoms and bonds in the tripeptide example are correct. Any help, please ? The following heavy (non-hydrogen) atoms are missing, which may result in inaccurate electrostatics: /A GLU 2 CG /A GLU 2 OE2 /A GLU 2 CA /A GLU 2 CD /A GLU 2 CB /A GLU 2 OE1 Using Amber 20 recommended default charges and atom types for standard residues Hydrogen /A LEU 3 HO bonded to atom that should not have hydrogens (/A LEU 3 OXT) -- doc. RNDr. Miroslav Iliaš, PhD. Katedra chémie Fakulta prírodných vied Univerzita Mateja Bela Tajovského 40 97401 Banská Bystrica tel: +421 48 446 7351 email : Miroslav.Ilias@umb.sk Miroslav Iliaš, PhD. Department of Chemistry Faculty of Natural Sciences Matej Bel University Tajovského 40 97401 Banska Bystrica Slovakia tel: +421 48 446 7351 email : Miroslav.Ilias@umb.sk