Without the details of what you did, I can only guess that somehow you did not enter the sequences correctly. They should be plain text, with only a comma between sequences of different proteins. see AlphaFold predict help: <https://rbvi.ucsf.edu/chimerax/docs/user/tools/alphafold.html#predict> To say anything more informative, we would need to see exactly what you pasted into the sequence area, and what options you chose in the input dialog. I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Jan 19, 2023, at 7:27 AM, Liang, Junyi via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:
I would like to predicate interaction between two proteins, and tried chimerax version 1.5 after noticing a youtube video https://www.youtube.com/watch?v=6lXeCPuTePs.
Running AlphaFold to Predict Protein Complexes from ChimeraX How to predict the structure of a photoreaction center complex of 3 proteins from ChimeraX using AlphaFold-Multimer. Requires a ChimeraX daily build newer than December 2, 2021 or version 1.4. It is not in ChimeraX version 1.3. Web page describing steps https://www.rbvi.ucsf.edu/chimerax/data/alphafold-dec2021/af_multimer.html www.youtube.com I input the two protein sequences in same way as shown in the video and hit predict. But your software says "missing or invalid sequences". How come?