Hi Nebojša, See the ChimeraX "volume" command "voxelSize" option: <https://rbvi.ucsf.edu/chimerax/docs/user/commands/volume.html#dimensions> The Chimera "volume" command has the same option: <https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html#dimensio...> ... and the Chimera Volume Viewer tool (GUI) has the voxel size setting in the Coordinates section (which you would show in the GUI using the Features menu): <https://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/volumeviewer/volu...> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Jul 28, 2022, at 6:38 AM, Nebojsa Bogdanovic via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:
Hello,
I have two maps. One from cryosparc @ 1.06 A/pix and another EM-gan sharpened map @ 1.0 A/pix. They are different in size upon visual inspection in chimeraX so I tried relion_image_handler --i cryosparc.mrc --angpix 1.0 --rescale_angpix 1.06 --o cryosparc-rescaled.mrc however, the resulting map is still smaller than the original input. I also tried to rescale in this way to several different angpix values but maps are still not the same.
Is there any command in chimeraX or chimera to play with pix sizes to find the right one?
Thank You and regards,
Nebojša Bogdanović, Ph.D Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University