Dear Eric, Thank you for your reply! I would also like to ask when I begin to Build Structure and create Gly-X-Gly peptides, there are multiple parameters that I could adjust such as Φ/Ψ with values for alpha helix/anti parallel beta strand/parallel beta strand/310 helix/pi helix and the rotamer library. I was wondering if just using the default values would be accurate enough? Could I also check when I measure SASA for the tripeptides, do I use the measure sasa /A:2 command or the info residues attribute area command as both give a different SASA value but from my understanding, the info residues attribute area gives a SASA value that represents the exposed area of the residue within the whole surface of the tripeptide while measure sasa command gives a SASA value that is representative of the area of that residue alone and hence, using info residues attribute area to obtain the reference SASA values is more accurate? Thank you so much! Warm regards, Nikhita