Hello,

I have recently watched a Youtube video (https://www.youtube.com/watch?v=GV6jHhxR5T4) about completing loops with Modeller in ChimeraX. However, the structure I want to model is only a part of the actual molecule, and I want to replace one unresolved segment (300 residues long) with 11 glycines.

When I upload both, the FASTA file with the sequence as it should look after completion of the loops, and the PDB file of the incomplete structure, I am not able to align them together in order to perform the modelling.

- If I use the original PDB file containing the SEQRES part, the association is performed but produces mismatches after the start of the unresolved sequence.

- If I use the PDB file without the SEQRES part, I am not able to perform the association.

Can anybody help me?

Thanks in advance

Sasha

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Alexandra Zahradníková, PhD

Department of Cell Cardiology

Institute of Experimental Endocrinology

Biomedical Research Centre SAS

Dúbravská cesta 9

845 05 Bratislava

Slovakia

Mobile: +421 908 069 796