Hi Melissa! Yes, ChimeraX can also be used for viewing larger objects like cells and organelles, e.g. from electron tomography or light microscopy. One issue may be the overall size of the data in terms of how many values rather than physical size, but even if the entire dataset is huge, there ways of handling it such as showing only a subsample (every Nth plane) and/or subregion (cropped or slabbed). The other issue is what input format(s) you have available, but ChimeraX reads a large number of volume formats including image stacks: <https://rbvi.ucsf.edu/chimerax/docs/user/commands/open.html#volume> I hope this helps, Elaine ----- Elaine C. Meng, Ph.D. UCSF Chimera(X) team Department of Pharmaceutical Chemistry University of California, San Francisco
On Jan 22, 2026, at 11:16 AM, Wilson, Melissa (NIH/NINDS) [F] via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:
Hello, I have a stack of serial electron microscopy images in which I segmented one single mitolysosme (basically a mitochondrion being degraded) across all the images in napari. My goal now is to create a 3D reconstruction of the mitolysosome from all the stacks. Napari hasn’t been working the way I’d like. I see a lot of protein structures used in your program. Can this program support visualization of larger structures –like this organelle? Sincerely, Melissa