Hi George,
The first thing to do is see if you can open and display your data files in ChimeraX. I don't know what formats you have for your EM map or your model. ChimeraX can read lots of map formats:
<https://rbvi.ucsf.edu/chimerax/docs/user/commands/open.html#volume>
As for your model, is it written out from IMOD? ChimeraX can read IMOD segmentations
<https://rbvi.ucsf.edu/chimerax/docs/user/commands/open.html#segmentation>
<https://rbvi.ucsf.edu/chimerax/docs/user/formats/imod.html>
Assuming you can read and display the data as you like, then the next thing is what you want in your movie. Do you just want it to rotate about the vertical axis once, or do you aspire to more complicated motions with multiple rotations and zooms, and/or playing through planes of the data or showing a sliding slab?
If it's just to rotate it around once, you could even try just clicking the movie-recorder icon in the top bar, which will automatically do that and save a movie file.
If you want the other fancier stuff, you would make a text file containing a series of ChimeraX commands and then open it to execute the commands. Command files:
<https://rbvi.ucsf.edu/chimerax/docs/user/commands/usageconventions.html#cxc-files>
Various movie-relevant ChimeraX commands are listed on our "making movies" page, along with links to example movie scripts.
<https://rbvi.ucsf.edu/chimerax/docs/user/movies.html>
For playing through the planes of data or showing a sliding slab, see also the "volume" command planes options:
<https://rbvi.ucsf.edu/chimerax/docs/user/commands/volume.html#planes>
Normally you would enter these commands interactively in the command line to try them out before even bothering to put them in your ChimeraX command file. Even after you start making a command file it is normally an iterative process of adding and modifying commands until the potential movie content satisfies you. Then finally as the last step you would bracket the other commands in the file with ones to start recording the movie and then at the end encode it into a movie file ("movie" command). Typically you would also save a session with your data displayed how you like it, so that in the future to reproduce the movie you would simply need to reopen the session and then open your previously written movie command script.
I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco
> On May 6, 2024, at 11:29 AM, George Schaible via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:
>
> Hello,
>
> My name is George Schaible and I am a post doc at Montana State University. I would like to use ChimeraX to make my movies of whole cell serial block face EM data I have modeled in IMOD/3dmod and I was wondering if someone could point me towards a tutorial or some way that I can make the movie using both the model and the original EM data. Any information is helpful and thank you!