Good morning UCSF ChimeraX Support Team, My name is Vincent Hsueh and I'm a P1 Pharmacy Student at Champan University. I'm a new member to the lab and it seems no one, currently with the lab, has prior experience to using ChimeraX. So far, I have isolated the Human ACE hAChE (PDB: 3LII) in the .csx file attached to the email. My PI gave me the task to superimpose to proteins, Human AChE (PDB: 3LII) colored in Beige and hTg ChEL (PDB 6SCJ) colored in Gold, but I'm having issues learning how to do it on my own. I'm trying to isolate Chain #B from PDB: 3LII with PDB: 6SCJ to see which part of 6SCJ is adjacent to 3LII prior to isolating both chains. I tried reading the ChimeraX user guide, and created the attached "in-progress(super-imposed)_final.csx file, but I'm still not sure how to do it properly. So far, I've opened my isolated PDB: 3LII model in ChimeraX, opened the PDB: 6SCJ file, used the matchmaker command, highlighted the corresponding sequences in SequenceViewer, but my PI has told me that the resulting model still has minor issues. Hopefully, I can gain some guidance in this effort to learn ChimeraX on my own, for my lab, and for future lab members! Vincent Hsueh PharmD Candidate Class of 2028 Chapman University School of Pharmacy vhsueh@chapman.edu<mailto:catvu@chapman.edu>
