5 Dec
2025
5 Dec
'25
10:23 p.m.
Dear all, Thanks for all your quick replies - this is super helpful! Tristan - thank you very much! I didn't know about that tool - I tried and it seems to have worked a treat. At least, my structure changed only minimally during Phenix refinement but the B-factors seem to have been overhauled. I may also compare this servalcat refinement - it sounds interesting! Regarding B-factors - I remember a long time ago there was some discussion about their relevance in cryo-EM: https://www.cell.com/structure/fulltext/S0969-2126(17)30246-0 . I have no idea what the prevailing thoughts in the field are now though! Thanks again, Louis