Hello,

My name is Yunsik Kang, and I am a postdoc in Marc Freeman’s lab at the Vollum Institute.

I would love to use ChimeraX to predict the structure of my protein of interest. I watched all the YouTube videos and tried to run the program.

Unfortunately, my protein is 5005 amino acids in humans and 2958 aa in yeast. I get a message “Please use the full AlphaFold system for long sequences.”

My question is what is the best way to approach this problem? Should I cut the protein in half and run the program? In one of the videos, it mentions after 700 aa it will have problems. Will it work if I get Colab-Pro? Or would the server crash no matter what.

I am not a structural biologist, but I hope the structure can help be predict me with my research.  

Thank you,

Yunsik